A NEW RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF DEFERIPRONE IN BULK AND ITS PHARMACEUTICAL DOSAGE FORM.

A simple, precise, new RP-HPLC method was developed and validated for the determination of Deferiprone in bulk and its pharmaceutical dosage form. In this method separation of liquid was done by using column C18, with mobile phase of water and Acetonitrile in the ratio of 55:45v/v ratio. The detection wavelength was found to be 280 nm with a flow rate of 1 ml/min and temperature of 300c. Retention time of Deferiprone was 4.960 min. The proposed method was validated as per standard guidelines. In the range of 10µg to 50µg/ml the linearity of Deferiprone shows a co-relation coefficient of 0.999. precision was found % RSD to be 0.70. The % mean recovery of Deferiprone was found to be 98.40%. The method was found to be robust even by changing in the flow rate of 0.2 ml/min and wavelength of ±2 nm .The developed method can be successfully employed for the routine analysis of Deferiprone in pharmaceutical dosage forms.