QUALITATIVE AND QUANTITATIVE ESTIMATION AND CHARACTERIZATION OF SECONDARY METABOLITES OF Typhonium trilobatum AND Trichosanthes dioica (Less focussed edible herbal medicinal plants of Bengal) LEAF EXTRACT

Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. So the aim of the present study is to investigate the two undocumented edible medicinal herb leaf Typhonium trilobatum (kharkol) and Trichosanthes dioica (parwal) for their qualitative and quatitative analysis and characterization of secondary metabolites which involves phytochemical screening assays, Total polyphenol content, Total flavonoid content, DPPH radical scavenging assay, ABTS radical scavenging assay, Total antioxidant capacity, Total tannin content and chromatographic techniques such as Column chromatography, HPLC and, TLC. Result of phytochemical sceening showed that, among different solvent extract most biologically active phytochemicals were present in 80% methanolic leaf extract of Trichosanthes dioica and Typhonium trilobatum, followed by aqueous extract irrespective of their content. Quantitative results revealed that both leaf extract were quite good enough in phytochemical and antioxidant potential but in comparison to Kharkol leaf Parwal leaf except total hydrolysable tannin, is a better source of polyphenol and flavonoids. Parwal leaf extract also exhibited more significant Total antioxidant capacity (387.84 µg/g dry extract), DPPH (50% inhibition at a concentration of 170.86 µg/ml) and ABTS (50% inhibition at a concentration of 140.64 µg/ml) radical scavenging potential than kharkol (170.01, 421.81 and 176.01 µg/ml respectively) leaf extract. HPLC analysis of purified fraction of both sample extracts documented that Quercetin, Gallic acid and in some cases Catechin was present as specific phytochemicals, which further support their disease treating potential in traditional medicine.