LIVER DYSFUNCTION OF WISTAR RATS INFLUENCED BY CITRIC ACID AS FOOD ADDITIVE.
Muawia I. A. Abdelgadir Elrrigieg
, Mahmood.M. Hassan
, Murwan K. Sabahelkhier
, Mohamed A. Adris
and Omer F. Idris.
- Department of chemistry, Faculty of Science, University of Bakht El – Ruda, Eld – Dueim, Sudan.
- Department of Biochemistry and molecular biology, Faculty of Fishery Sciences and Technology, El Neelain University, Khartoum, Sudan.
- Department of Biochemistry and molecular biology, Faculty of Science and Technology, El Neelain University, Khartoum, Sudan.
- Department of Biochemistry and molecular biology, Faculty of Medicine, Dungla University, Dungla, Sudan.
- Abstract
- Keywords
- Cite This Article as
- Corresponding Author
The aim of this study was focused on assessing liver function of Wistar rats influenced by oral ingestion of different doses of the food additive citric acid, using rats' serum AST as an indicator. Administered doses were (100, 600 and 1250 mg/kg b.w). 36 female Wistar rats were divided into 4 groups (9 rats /each). One group served as control, and the remainder of the 3 groups received different doses of citric acid. Housing was in Meck Nimir Research Center, Khartoum. Animals were accessed to free tab water and standard diets liberally. Serum was taken from each rats and analyzed by spectrophotometer. Transverse sections of liver organs were used to prepare Histopathiological slides. A significant (p ? 0.05) gradual increase according to increased treatment doses in serum AST was observed in treated animals, compared with control. Also there were different signs of liver histopathological abnormalities including atrophy (black material inside kupfler cell), Necrosis, hyperaemia and deattached cells, changes mainly around central vein, infiltration of black material, small particles, hyperaemia, cellular damage, cytoplasmic vacuolation, and polymorphic nuclei, compared to control. All tested animals showed significant (p ? 0.05) inccreased body weight, compared to control. All animals survived till the end of the experiment. As a conclusion we can state that oral ingestion of citric acid results in liver dysfunction, so it is preferred to ingest foods and beverages containing citric acid with caution. This study was aimed to assess rats’ liver dysfunction influenced by oral ingestion of citric acid..
[Muawia I. A. Abdelgadir Elrrigieg, Mahmood.M. Hassan , Murwan K. Sabahelkhier, Mohamed A. Adris and Omer F. Idris. (2016); LIVER DYSFUNCTION OF WISTAR RATS INFLUENCED BY CITRIC ACID AS FOOD ADDITIVE. Int. J. of Adv. Res. 4 (Jun). 2085-2089] (ISSN 2320-5407). www.journalijar.com
