The Effect of IL-10 Polymorphism in Patients withNon Hodgkin’s Lymphoma in Iraq.

This study aims to examine the association of single nucleotide polymorphisms (SNPs) of IL-10-1082A/G with the incidence of NHLs in patients in Iraq.Fifty-five confirmed patients with NHLs and 40 family-unrelated, age-matched apparently healthy controls were used for blood and DNA extraction. Allele specific polymerase chain reaction (AS-PCR) technique was used for genotyping of the IL-10 SNP.IL-10 production levels in sera of patients and healthy individualswere measured by using ELISA.The SNPIL-10-1082A/G appeared in three genotypes: AA, AG and GG. These genotypes represented 36.36%, 45.45% and 18.18%%, respectively, in NHL patients compared to 32.5%, 35% and 32.5%% in healthy controls, respectively. There was no significant correlation between neither genotypes nor alleles and NHL incidence. IL-10 levels in NHL patient sera was significantly higher (t=2.606, P=0.011) than that of healthy control but is neither linked to IL-10 genotypes nor with allele distribution frequency.These results demonstrated that IL-10-1082A/G polymorphism has no significant effect on the incidence of NHLs,therefore other risk factors could be examined further. These suggest that multiple factors contribute to the susceptibility of NHLs in Iraqi patients.