Molecular analysis of prolactin gene exons region of hyperprolactinemic and breast cancer Iraqi patients

This study was constructed to investigate hyperprolactinemia related infertility through the molecular base associated with single nucleotide polymorphism (SNP) at prolactin gene in hyperprolactemic patients with the breast cancer risk. This study included one hundred fifty blood samples from patients suffering hyperprolactinemia and infertility. Also twenty five tissue samples of breast cancer patients were collected in which fifteen samples were frozen tissue and ten samples were formalin fixed paraffin embedded tissue. Fifty blood samples from healthy persons were collected served as control group. The main ages of patients were 20 to 50 and same for control (healthy) group. it?s clear that there is substitution and deletion mutation, in which the highest mutation number was in exon 2, which was 9 mutations, all mutations in this exon was substitution ,while the less mutation number was in exon 3 and exon4 which was 2 for each exon, one substitution and one deletion mutation in exon 3 while the two mutations in exon 4 was substitution only. The risk association between the DNA of hyperprolactemic and breast cancer patients using information on national center for biotechnology information (NCBI), and Mega 6 program.