Determination of some derivative amine with Bethanechol by Ion Chromatography

Amines and derivative amine Compound are detected by Ion Chromatography measuring Detected at baseline 0.15µs cm-1 of complex formed with the post column Methane sulphonic acid as eluent .Using this method , derivative amine will coelunt with the Bethanechol. Rapid, efficient, cost effective and reproducible isocratic reversed phase method has been developed and validated for the determination of Bethanechol in active an organic ions using mobile phase 30mM Methane sulphonic acid having pH 4.5 Mobile phase was pumped at a flow rate of 0.25 mL min?1 using isocratic elution through Ion pac ,Cs18 (250×2 mm, 5 ?m) column. Conductivity detection was performed at 0.15µs cm-1 . Method was validated following the IC Isocratic. Calibration curve was linear in concentration range [Diethyl amine (A)=0.2-1.0 , Ethylene diamine (B)=0.1-0.5 , Trimethyl amine (C)=0.3-1.0 ), bethanechol chloride (D)=5-25] ?g mL?1 ( n=3) with correlation coefficient 0.996-0.999, and lower limits of detection and quantitation as 1.050 ,0.529 ,1.495 and ,26.400 ng mL?1 and 3.184 ,1.605 4.532 and 80.000 ng mL?1 in standard material respectively. Recovery was found to be in the range 97-100% and precision less than 1%. Developed method was successfully applied for analytic analysis of standard solution and also to study the interaction of Derivative Amine and Bethanechol temperature (25-30°C) .