COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFICACY OF SODIUM HYPOCHLORITE, CHLORHEXIDINE, NEEM AND GREEN TEA AGAINST E. FAECALIS - AN IN VITRO STUDY.
- Abstract
- Keywords
- References
- Cite This Article as
- Corresponding Author
Aim and Objectives: To check the antimicrobial efficacy of sodium hypochlorite (NaOCl), chlorhexidine (CHX), neem and green tea against E. faecalis. Materials and Methods: The irrigants were divided into four groups, Group I- NaOCl, Group II- CHX, Group III- Neem, Group IV- Green tea. Agar well diffusion test was used to study the antimicrobial efficacy against E. faecalis. The plate was incubated at 37?C for 24hrs in an incubator. The zone of inhibition was recorded, tabulated, and analyzed statistically using analysis of variance. Results: All four irrigants showed well-defined and comparable zones of inhibition around their respective wells. There was significant difference between zone diameters of CHX, neem, and NaOCl against E. faecalis(p < 0.05). Conclusion: From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and sodium hypochlorite.
- Narayanan LL, Vaishnavi C. Endodontic microbiology. J Conserv Dent2010;13(4):233?9.
- Bystr?m A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res 1981;89:321‑
- Gomes BP, Souza SF, Ferraz CC, Teixeira FB, Zaia AA, Valdrighi L, et al. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalisin bovine root dentine in vitro. IntEndod J 2003;36:267‑
- Silva FB, Almeida JM, Sousa SM. Natural medicaments in endodontics ? A comparative study of the anti‑inflammatory action. Braz Oral Res 2004;18:174‑
- McComb D, Smith DC. A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod 1975;1:238‑
- Oncag O, Cogulu D, Uzel A, Sorkun K. Efficacy of propolis as an intracanal medicament against Enterococcus faecalis. Gen Dent 2006;54:319‑
- Hugo WB, Longworth AR. Some aspects of the mode of action of chlorhexidine. J Pharm Pharmacol 1964;16:655‑
- Love RM. Enterococcus faecalis: A mechanism for its role in endodontic failure. IntEndod J 2001;34:399‑
- Parle M, Bansal N. Herbal medicines: Are they safe? Nat Prod Radiance 2006;5:6‑
- Prabhakar J, Senthilkumar M, Priya MS, Mahalakshmi K, Sehgal PK, Sukumaran VG. Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and green tea polyphenols), MTAD, and 5% sodium hypochlorite against Enterococcus faecalisbiofilm formed on tooth substrate: An in vitro J Endod 2010;36:83‑6.
- Karimi K. The impact of current alternative herbal remedies on dental patient management. Gen Dent 1999;47:264‑
- Bohora A, Hegde V, Kokate S. Comparison of antibacterial efficacy of neem leaf extract and 2% sodium hypochlorite against faecalis, C. albicansand mixed culture ? An in vitro study. Endodontology 2010;22:8‑12.
- Hamilton‑Miller JM. Anti‑cariogenic properties of tea (Camellia sinensis). J Med Microbiol 2001;50:299‑
- Smullen J, Koutsou GA, Foster HA, Zumb? A, Storey DM. The antibacterial activity of plant extracts containing polyphenols against Streptococcus mutans. Caries Res 2007;41:342‑
- Castilho AL, Saraceni CH, D?az IE, Paciencia ML, Suffredini IB. New trends in dentistry: plant extracts against Enterococcus faecalis. The efficacy compared to chlorhexidine. Braz Oral Res 2013;27(2):109-115.
- Distel JW, Hatton JF, Gillespie MJ. Biofilm formation in medicated root canals. J Endod 2002;28:689‑93.
- Vatkar NA, Hegde V, Sathe S. Vitality of Enterococcus faecalis inside dentinal tubules after five root canal disinfection methods. J Conserv Dent 2016,19:445-9.
- Gupta N, Singh N, Thaper B. Effect of addtion of 2% chlorhexhidinegluconate, clcium hydroxide, and tetracyclin powder on antimicrobial activity of mineral trioxide aggregate. Endodontology 2016;28:23-6.
- Sundaram, D., Retna, K.N. and Kannan, V. A comparative evaluation on antimicrobial effect of honey, neem leaf extract and sodium hypochlorite as intracanalirrigant: an ex-vivo study. J ClinDiagn Res 2016:10.
- Martina L, Ebenezar A, Ghani M. An in vitro comparative antibacterial study of different concentrations of green tea extracts and 2% chlorhexidine on Enterococcus faecalis. SEJ 2013;3: 120?123.
- Singh H, Kaur M, Dhillon JS, Batra M, Khurana J. Neem: a magical herb in endodontics. Stomatological Dis Sci 2017;1:50-4.
- Bhargava K, Kumar T, Aggarwal S, Zinzarde S, Sanap A, Patil P. Comparative evaluation of the antimicrobial efficacy of neem, green tea, triphala and sodium hypochlorite: An in vitro study. J Dent Res Rev 2015;2:13-6.
[Vrishali Shrigondekar, Jyoti Pawar, Meenal Gulve, Roshan Samuel, Swapnil Kolhe And Gayatri Aher. (2019); COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFICACY OF SODIUM HYPOCHLORITE, CHLORHEXIDINE, NEEM AND GREEN TEA AGAINST E. FAECALIS - AN IN VITRO STUDY. Int. J. of Adv. Res. 7 (Apr). 449-453] (ISSN 2320-5407). www.journalijar.com
M.G.V'S K.B.H DENTAL COLLEGE AND HOSPITAL, PANCHAVATI, NASHIK, MAHARASHTRA