15Nov 2017

INHIBITORY EFFECT OF THE ACETONE EXTRACT OF MAIZE LEAVES ON THE MYCELIAL GROWTH OF ASPERGILLUS PARASITICUS ALONG WITH ITS AFLATOXINS PRODUCTION.

  • Maneesh Kumar, Harish Kumar, Roshan Kamal Topno, Ganesh Chandra Sahoo, Subham Kumar, Kundan Kumar, Siddharth Jha and Sushil Kumar.
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This study shows the anti-fungal and anti-aflatoxigenic activities of acetone extract of Maize leaves. As contamination caused by Aspergillus parasiticus and aflatoxin is a major challenge in agriculture and food industry, we tried to inhibit the mycelial growth of A. parasiticus and aflatoxins production. Here, we investigated the effect of acetone extract of hybrid stain of maize leaves (Malviya Hybrid Makka) on the growth of A. parasiticus and aflatoxin production. Phytochemical screening of this extract was also carried out which revealed the presence of many anti-fungal and anti-aflatoxigenic compounds such as proteins, carbohydrates, flavonoids and terpenes. The extract showed 82.89% inhibition of the growth of A. parasiticus mycelial and 100% inhibition of the aflatoxins production at the concentration of 250?l/ml and 200?l/ml respectively. TLC method was used for qualitative and quantitative analysis of the aflatoxins that revealed the bands of aflatoxin B1, B2, G1 and G2, and indicated that B1 is found in higher concentration than other aflatoxins. The overall study indicates that acetone extract of Maize leaves possess good potential to arrest the growth of A. parasiticus mycelial and its aflatoxins production.


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[Maneesh Kumar, Harish Kumar, Subham Kumar, Kundan Kumar, Siddharth Jha and Sushil Kumar (2017); INHIBITORY EFFECT OF THE ACETONE EXTRACT OF MAIZE LEAVES ON THE MYCELIAL GROWTH OF ASPERGILLUS PARASITICUS ALONG WITH ITS AFLATOXINS PRODUCTION. Int. J. of Adv. Res. 5 (Nov). 751-757] (ISSN 2320-5407). www.journalijar.com


Maneesh Kumar
Department of Virology, Rajendra Memorial Research Institute of Medical Sciences (ICMR), Agam kuan, Patna-800007, Bihar, India.

DOI:


Article DOI: 10.21474/IJAR01/5827      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5827