15Aug 2017

SOLID STATE FERMENTATION OF WHEAT STRAW FOR PRODUCTION OF MNP BY P. CHRYSOPORIUM MTCC 787.

  • Department of Microbiology, Shree M. & N. Virani Science College, Rajkot, Gujarat, India. 360005.
  • School of Life Sciences, Central university of Gujarat, Gandhinagar, Gujarat, India. 382030.
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Solid state fermentation of wheat straw was attempted with P. chrysosporium MTCC 787 for production of MnP at 39?C. Fermented residue was extracted with 0.2 M sodium tartarate buffer (pH 3) and was subjected to ammonium sulfate precipitation. Precipitates obtained were subjected to MnP assay, upon dialysis, using MBTH and DMAB as substrate. Optimum pH and temperature were reported to be pH 4.5 and 30?C respectively. Km and Vmax of MnP for MBTH were found to be 0.05 mM and 25 U/mg, respectively. Enzyme kinetics were also assessed against Reactive Black B (RBB), a widely used textile diazo dye, as substrate. Km and Vmax of MnP for RBB were reported as 0.2 mM and 7 U/mg, respectively. These findings suggest enormous potential of MnP of P. chrysosporium MTCC 787 for its application in treatment of RBB containing wastewater.

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[Nishant Junnarkar and Neepa Pandhi. (2017); SOLID STATE FERMENTATION OF WHEAT STRAW FOR PRODUCTION OF MNP BY P. CHRYSOPORIUM MTCC 787. Int. J. of Adv. Res. 5 (Aug). 852-857] (ISSN 2320-5407). www.journalijar.com

NISHANT JUNNARKAR
DEPARTMENT OF MICROBIOLOGY, SHREE M. & N. VIRANI SCIENCE COLLEGE, RAJKOT, GUJARAT, INDIA. 360005

DOI:

Article DOI: 10.21474/IJAR01/5128      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5128

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