Effect of culture media on biosynthesis of titanium dioxide nanoparticles using Lactobacillus crispatus

The present study included the rapid synthesis of Titanium dioxide nanoparticles using Lactobacillus crispatus and focus on the effect of different culture media (MRS broth,JKH1 broth and Whey solution) on TiO2 nanoparticles synthesis . The synthesized nanoparticles were characterized using X-ray diffraction (XRD) technique, Atomic Force Microscopy(AFM) and Scanning electron microscopic(SEM). The XRD pattern of the sample showed the presence of main peak of ?=25.3 matches the (101) crystallographic plane of anatase of TiO2 nanoparticles .The morphological characteristics were found to be spherical, oval in shape, have the average size (70.98 ,116.89, 113.67)nm synthesized in MRS broth, JKH1 broth and whey solution respectively. Adding of 10% glucose to JKH1 broth and Whey solution lead up to decreasing the average size of synthesized nanoparticles reached to (92.65 and 112.26)nm for JKH1 broth and whey solution respectively.