21Jun 2017

STUDY ONMICROBIAL DIVERSITY OF BIOSURFACTANT PRODUCING BACTERIA FROM CONTAMINATED ENVIROMENTAL SAMPLES.

  • Environmental Biology Dept, Federal Polytechnic Nekede Owerri, Imo-State.
  • Biotechnology Dept, Federal University of Technology Owerri, Imo-State.
  • Chemistry Dept, Federal University of Technology Owerri, Imo-State.
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Through culture enrichments we isolated nine bacterial genera: Staphylococcus aureus 9.1 %, Bacillus sp. 21.2%, Pseudomonas aeruginosa 27.3%, Corynebacterium spp., 9.1%, Streptococcus spp., 9.1%, Micrococcus spp., 12.1%, Enterobacter spp., 3.0%, Proteus spp., 6.1%, Escherichia coli 3.0% from numerous contaminated soils and water samples We characterized the bacterial strains that can utilize different hydrocarbons (diesel, petrol, kerosene, crude oil, vegetable oil) using βhaemolysis, methylene blue plate assay, drop collapse test, oil displacement and emulsification index; 16 isolates (66.7%) produced βhaemolysis, 6 isolates (25%) positive for methylene blue assay, 16 isolates (66.7%) collapsed in drop collapsing test. The oil spreading test on diesel, N8 (85 mm, Bacillus sp.) and N12 (80 mm, Pseudomonas sp.) displayed highest values. The emulsification index on diesel N22 (71 mm, Proteus sp), N7 (67 mm, Pseudomonas sp.) recorded the highest. All strains tested, emulsified the oil to varying degrees and their ability to produce biosurfactant ascertained.

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[C. C. Okore, L. U. Nwaehiri, O.N. Mbanefo, T. E. Ogbulie, A. U. Ugenyi, I. B. Ogbuka, A. E. Ejele and I. A. Okwujiako. (2017); STUDY ONMICROBIAL DIVERSITY OF BIOSURFACTANT PRODUCING BACTERIA FROM CONTAMINATED ENVIROMENTAL SAMPLES. Int. J. of Adv. Res. 5 (Jun). 1387-1396] (ISSN 2320-5407). www.journalijar.com

CHIOMA C. OKORE
FEDERAL POLYTECHNIC NEKEDE OWERRI, IMO-STATE, NIGERIA

DOI:

Article DOI: 10.21474/IJAR01/4548      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4548

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