31May 2017

INDUCTION OF BIOCHEMICAL CHANGES IN MOUSE TESTIS AFTER THE COMBINED TREATMENT OF RADIATION AND CADMIUM CHLORIDE.

  • Department of Zoology, Radiation Biology Laboratory, Govt. Lohia College Churu, India.
  • Department of Zoology, Radiation Biology Laboratory, Govt. Dungar College Bikaner, India.
  • Former Joint Director, College Education, Rajasthan, India.
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Background: The main objective of the present investigation was to observe biochemical changes induced by radiation and cadmium individually as well as simultaneously on the testis of Swiss albino mice. Materials and Methods: For this purpose, adult male Swiss albino mice (6-8 weeks old) were divided into four groups. Group I (sham-irradiated), Group II (treated with CdCl2 solution 20 ppm), Group III (irradiated with 1.25, 2.5 and 5.0 Gy gamma rays), Group IV (both irradiated with 1.25, 2.5 and 5.0 Gy gamma rays and treated with CdCl2 solution). The animals were autopsied after 1, 2, 4, 7, 10, 14 and 28 days of treatment. The testis was taken out and different biochemical parameters, such as total proteins, DNA and RNA were estimated. Results: It was found that contents of Total proteins, DNA and RNA decreased. Such decline was observed with higher dose (5.00 Gy) at early intervals. Recovery started at day 10 or 14 but control level could not be attained up to the last intervals. A similar decreasing pattern was observed with 2.5 and 1.25 Gy, but it was found lesser as compared to 5.0 Gy group. The recovery was also faster in these groups. Results indicated the infliction of radiation insults and subsequent repair in the testes to be dose-dependent. In combined treatment group the pattern of biochemical changes was similar to individual treatment but the magnitude of occurrence was statistically higher and the normal value could not be attained even at last autopsy (28 days). Thus the damage and recovery pattern in combined treatment (radiation and cadmium chloride) indicates the ?synergistic? effect of these two agents. Conclusion: These results indicate that combined treatment of cadmium and gamma radiation causes synergistic or additive effect.

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[C. L. Verma, R. K. Purohit and M. L. Gupta. (2017); INDUCTION OF BIOCHEMICAL CHANGES IN MOUSE TESTIS AFTER THE COMBINED TREATMENT OF RADIATION AND CADMIUM CHLORIDE. Int. J. of Adv. Res. 5 (May). 2195-2200] (ISSN 2320-5407). www.journalijar.com

Dr. C. L. Verma
Radiation Biology Laboratory, Department of Zoology, Govt. Lohia College, Churu, India -331001

DOI:

Article DOI: 10.21474/IJAR01/4368      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4368

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