28May 2017

A NEW DIAGNOSTIC METHOD TO DETECT BACTEREMIA TARGETING 23S AND 16S rRNA SEQUENCES BY STANDARD PCR

  • Research Scholar Dept. of Biotechnology Dr.V.S.Krishna Govt. College(A), Affiliated to Andhra University.
  • Research guide & Director Centre for Research Studies.Dr.V.S.Krishna, Govt. College(A) Affiliated to Andhra University.
  • P.G. Dept of Biotechnology, Dr.V.S.KrishnaGovt.College(A).
  • Dean Biotechnology A.P.IIIT-IdupulapayaKadapa&Nuzvid.
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Background: The rapid and accurate detection of bacteremia might have a significant impact in shortening hospital stays within the intensive care unit (ICU) as well as reducing the costs to the health care system. PCR primers targeted to conserved rRNA gene sequences allowed the RNA amplification of virtually any bacterial species. Objective:The aim of this study is to establish a new diagnostic method to detect bacteremia, using universal primers targeted to 23S rRNA and 16SrRNA, directly from blood culture plates.To some extent to address the Nosocomial infections. Materials & Methods: Phase I study is doneby doing BLAST search, in which organisms like E.coli, Staphylococcus aureus, Klebsiellapneumoniae, Pseudomonas aeroginosa showed 100% sequence similarity with the primers targeted to 16S and 23S rRNA. Results:Out of 10 samples, five were identified as E.coli, two were identified as Staphylococcus, two were identified as Klebsiella and one was identified as Pseudomonas. All the bacterial colonies gave 16S and 23S rRNA amplicons of 1404bp and 896bp except one sample which was identified as E.coli by biochemical analysis. Conclusion:The 23S rRNA sequences targeted are conserved, so gave 100% results upon amplification but the 16S rRNA sequences gave only 90% results when used on same organisms as more heterogenicity was found.

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[K. Bramarambica, Y. V. K. Durga Prasad, B. Priyanka and S. Harasreeramulu. (2017); A NEW DIAGNOSTIC METHOD TO DETECT BACTEREMIA TARGETING 23S AND 16S rRNA SEQUENCES BY STANDARD PCR Int. J. of Adv. Res. 5 (May). 1524-1531] (ISSN 2320-5407). www.journalijar.com

Dr.K.Bramarambica
Research Scholar Dept. of Biotechnology Dr.V.S.Krishna Govt. College(A) Affiliated to Andhra University

DOI:

Article DOI: 10.21474/IJAR01/4276      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4276

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