30Mar 2017

Do Gamma Rays of Cancer Radiotherapy Effect on the Sequence of Collagenase Gene?

  • Department of biological Applications, Nuclear Research Center, Atomic Energy Authority, Egypt.
  • Department of Zoology, Faculty of Science, Benha University, Egypt.
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Background: expression of certain HLA alleles, considered suscepti-ble factor to acute leukemia (AML, ALL), there were clear associations between resistance or susceptibility to cancer and (HLA) profile, this work aims to investigate role HLA-DRB1 and anti HLA?G in development of AML. Patients and methods: (40) patients with acute leukemia aged from 4 to 60 years (23 male and 17 female), and 20 healthy subjects as a control, all were tested for anti-HLA-G by four-color flow cytometer using FACS Canto(BD Biosciences: USA), HLA-DRB1by Sequence Specific Oligonucleotide PCR (SSO-PCR), DNA purification by (Spin Protocol), Qiagen, Hilden, Germany, QIA amp DNA Mini, thermal cyclers BIO-RAD PTC-100 (USA), hybridization with DNA probes, alleles detection; INNO-LiPA HLA typing, Fuji Rebio Group, Belgium, with the INNO-LiPA HLA-DRB1 Plus kit, Cat. No. #58355. Statistical analysis: SPSS 19 edition was used, gene frequencies of HLA-DRB 1alleles were calculated by direct count. Results: there is an allelic association between HLA-DRB1 and leukemia, patients and control showed significant difference between DRB1*13 (p = 0.006 and p = 0.016 respectively) (protective nature), but there were moderate difference among DRB1*06 (p = 0.098), DRB1*14 (p = 0.098), (also, protective nature) and *15 (p = 0.098) (Risk factor). However, we did not observe any significant difference for the others HLA-DRB1. Conclusion: HLA-DRB1*13, and HLA-DRB1*15 alleles were protective while HLA-DRB*06 and DRB1*14 were predisposing factor for AML.

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[Amr M. Mousa, Mohammed H. Awwad, Samia. E. Ali, Abdelmonsef A. Elhadary and Omar I. Ghonemy. (2017); Do Gamma Rays of Cancer Radiotherapy Effect on the Sequence of Collagenase Gene? Int. J. of Adv. Res. 5 (Mar). 1811-1818] (ISSN 2320-5407). www.journalijar.com

Hasnaa A Abo-Elwafa
Assistant Prof. of Clinical Pathology, Faculty of Medicine, Sohag University, Egypt

DOI:

Article DOI: 10.21474/IJAR01/3696      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3696

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