30Jan 2017

CONTROL OF ENVIRONMENTAL POLLUTION BY EFFECTIVE UTILIZATION OF FLY-ASH PRODUCED BY THERMAL POWER PLANTS IN CULTIVATED LAND AND BIODEGRADATION OF PLASTIC USING PSEUDOMONAS SP. ISOLATED FROM WASTE WATER

  • Department of Biotechnology, Panskura Banamali College. West-Bengal. India.
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Accumulation of plastics in the environment is creating a threat to the mankind. Some plastics are degradable which do not cause threat to the environment, and are being used for several purposes such as making of containers, use for packaging, water pipes etc. In this study, the isolation and characterization of plastic degrading bacteria from paper mill waste water was done. Identification of the isolated strains was performed on the basis of colony morphology, grams nature, and several biochemical tests. Plastic strips are subjected to biodegradation by isolated bacteria using mineral salt medium. The degradation was observed by changes in physical and optical characteristics. The maximum degradation was observed in Pseudomonas sp. showed after 21 days of incubation Utilization of fly-ash, produced by thermal power plants in different fields to control environmental pollution has given due importance for the last many years. In this paper an attempt has been made to present the results of some experiments conducted to utilize fly-ash collected from Kolaghat thermal power plant, properly by extracting its suitable nutrients, by Pseudomonas sp. isolated from waste water, useful for plant growth. To conduct this experiment, a single bacterial isolate are isolated from waste water through King’s media. After single colonies isolation and thrice time purification, isolate are stored in 40% glycerol solution at isolate are separated for studying their fly-ash and their seed germination as well as plant growth promoting ability on isolate showed good results of seed treatment whether on general soil or fly have been in tabulated form.


  1. Chand N, Vashishtha SR ,“Development, Structure and Strength Properties of PP/PMMA/FA Blends”. Mater. Sci., (2000) 23(2), 103-107.
  2. Steveson M, Sagoe-Crentsil K, “Relationship Between Composition, Structure and Strength of Inorganic Polymers, Part 2: Fly Ash-Derived Inorganic Polymers”. Matter. Sci.,(2005) 40(16), 4247-4259.
  3. Paul KT, Satpathy SK, Manna I, Chakroborty KK, Nando GB ,“Preparation and Characterization of Nano Structured Materials from Fly Ash: A Waste from Thermal Power Stations, by High Energy Ball Milling”. Nanoscale Res. Lett.,(2007) 2(8), 397-404.
  4. Usha R, Sangeetha T and Palaniswamy M, Libyan Agric Res Center J I nternati (2011)2(4), 200-204.
  5. Shimao M. Curr. Opin. Biotechnol ,(2001) 12, 242-247.
  6. Bellina G, Tosin M, Degli-Innocenti F. Polym Degrad Stab (2000) 69, 113–120.
  7. Pometto, AL; Lee, B; Johnson, KE, Production of an Extracellular Polyethylene- Degrading Enzyme(s) by Streptomyces Species. Appl. Environ. Microbiol,(1992) 58 (2), 731 - 733.
  8. Starnecker, A; Menner, M, Assessment of biodegradability of plastics under stimulated composting conditions in a laboratory test system.
  9. Biodeterior. Biodegrad(1996), 85 - 92.
  10. Gu, JD; Ford, TE; Mitton, DB; Mitchell, R (2000). Microbial corrosion of metals. The Uhlig Corrosion Handbook. 2nd Edition. Wiley, New York, USA.
  11. Wang YZ, Yang KK, Wang XL, Zhou Q, Zheng CY, Chen ZF.J Poly Environ,(2004)12, 7-11.
  12. Sharma Prabhat, Bhattacharyya S, Verma Vishal, Kalyani RK, Kumar Vijai, Pandey KN and Singh M. Internat Res J Environ Sciences,( 2013)2(9), 83-85.
  13. Premraj R,Dan Mukesh Doble. Ind J Biotech, (2005) 4,186-193.
  14. Priyanka N. Internation J of Biomed Ad Res,( 2011)2(9), 235-238.
  15. Khan AA, Jilani G, Akhtar MS, Naqvi SMS, Rasheed M , “Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and Their Role in Crop Production”. Agric. Boil. Sci, (2009) 1 ,48-58.
  16. Mahdi SS, Hassan GI, Hussain A, Rasool F (2011). “Phosphorus Availability Issue- Its Fixation and Role of Phosphate Solubilizing Bacteria in Phosphate Solubilization”. J. Agric. Sci, (2011) 2 , 174-179.
  17. King EO, Ward MK, Raney DE, “Two Simple Media for the Demonstration of Pyocyanin and Fluorescein”. Lab. and Clinical Microbiol,(1954) 44, 301-307.
  18. Bennett PC, Rogers JR, Choi WJ, “Silicates, Silicate Weathering, and Microbial Ecology”. Geomicrobiology Journal,(2001) 18 , 3-19.
  19. Giere R, Carleton LE, Lumpkin GR , “Micro- and Nano-Chemistry of Fly Ash from Coal-Fired Power Plant”. Mineral,(2003) 88,1853.
  20. Sadasivan S, Negi BS, “Chemical Characteristics of Fly Ash from Coal Fired Thermal Power Plants in India”, Science of the Total Environment, (1991)103 ,151-158.
  21. Khandkar UR, Gangwar MS, Srivastava PC, Singh M (1993). “Edaphological Characteristics of Unwealthered and Welathered Fly Ashes from Gondwana and Lignite Coal”, Environment Pollution.
  22. Jayaprakashvel M, Muthezhilan R, Srinivasan R, Hussain AJ, Gobalakrishnan S, Bhagat J, Kaarthikeyan C and Muthulakshmi R, “Hydrogen Cyanide Mediated Biocontrol Potential of Pseudomonas sp”. AMET1055 Isolated from the Rhizosphere of Coastal Sand Dune Vegetation, (2010)10, 39-42.
  23. Nishida H, Tokiwa Y. J Environ Polym Degrad, (1998)1, 227–233.
  24. El-Shafei, HA; El-Nasser, NHA; Kansoh, AL; Ali, AM, Biodegradation of disposable polyethylene by fungi and Streptomyces Polym. Degrad. Stab,(1998) 62, 361 - 365.
  25. Abdul Baki A A, Anederson J D , “Vigour Derermination in Soybean Seed by Multiple Criteria”. Crop Sci,(1973) 13 , 630-633.
  26. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994). “Bergey’s Manual of Determinative Bacteriology”, 9th Edition Published by Williams and Wilkins, A. Wavely Company, Baltimore.

[Somnath De, Nargis Parvin, Moumita Banerjee, Tapas Giri, Sk Iqbal Hossain, Mirza Mehedi Hasan and Subho Panja. (2017); CONTROL OF ENVIRONMENTAL POLLUTION BY EFFECTIVE UTILIZATION OF FLY-ASH PRODUCED BY THERMAL POWER PLANTS IN CULTIVATED LAND AND BIODEGRADATION OF PLASTIC USING PSEUDOMONAS SP. ISOLATED FROM WASTE WATER Int. J. of Adv. Res. 5 (Jan). 1919-1925] (ISSN 2320-5407). www.journalijar.com


Somnath De
Department of Biotechnology, Panskura Banamali College. West-Bengal. India.

DOI:


Article DOI: 10.21474/IJAR01/2961      
DOI URL: http://dx.doi.org/10.21474/IJAR01/2961