20Jan 2017

COMPLETE NUCLEOTIDE SEQUENCE OF A MONOPARTITE TOMATO LEAF CURL NEW DELHI VIRUS AND BETASATELLITE DNA ASSOCIATED WITH LEAF CURL DISEASE OF TOMATO IN INDIA.

  • School of Life Sciences, Jawaharlal Nehru University, New Mahrauli Road, New Delhi-110067, India.
  • Dept. of Biotechnology, School of Sciences, Noida International University, GB Nagar, Greater Noida, Uttar Pradesh, India.
  • Centre for research studies, Noida International University, GB Nagar, Greater Noida, Uttar Pradesh, India.
  • University of Kashmir Hazratbal, Srinagar, Jammu and Kashmir- 190006, India
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A begomovirus was isolated from tomato plants showing typical leaf curl and stunting symptoms in tomato fields near the Delhi, India. Viral genomic components were enriched by rolling-circle amplification; PCR amplified and were cloned and sequenced. The genome organization of this virus was found to be similar to those of Old World monopartite begomovirus, with DNA-A and a betasatellite component. The alphasatellite or DNA-B component was not detected. The complete nucleotide sequence of DNA-A shared highest similarity with Tomato leaf curl New Delhi (ToLCNDV) virus while betasatellite DNA showed similarity with betasatellite DNA reported from infected leaves of Tomato, Sunflower and Potato. The data present here strongly support the association of a monopartite ToLCNDV and betasatellite DNA with the leaf curl disease of tomato.

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[Abhinav Kumar, Taruna Gupta, Seema Singh and Neera B. Sarin. (2017); COMPLETE NUCLEOTIDE SEQUENCE OF A MONOPARTITE TOMATO LEAF CURL NEW DELHI VIRUS AND BETASATELLITE DNA ASSOCIATED WITH LEAF CURL DISEASE OF TOMATO IN INDIA. Int. J. of Adv. Res. 5 (Jan). 1683-1686] (ISSN 2320-5407). www.journalijar.com

ABHINAV KUMAR
NOIDA INTERNATIONAL UNIVERSITY

DOI:

Article DOI: 10.21474/IJAR01/2937      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2937

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