GLYCOCONJUGATES OF RAT LUNGS STRUCTURAL COMPONENTS ACCORDING TO LECTIN HISTOCHEMICAL STUDIES DURING EXPERIMENTAL HYPOTHYROIDISM.

Glycoconjugates of rat lungs structural components according to lectin histochemical studies during experimental hypothyroidism Antonina Yashchenko, Lesia Pankevych, Olena Smolkova, Alexander Lutsyk, Khrystyna Strus, Igor Zastavnyy The experimental studies involved 20 sexually mature male rats 180-200 g. weight, including 10 control and 10 with experimental hypothyroidism induced by the introduction into the food thyreostatic drug merkazolilum at a dose of 5 mg / kg body weight for 30 days. Histological material was fixed in 4% neutral formalin. Overview slides 5-7 ?m thick were stained by hematoxylin and eosin. Glycoconjugates of the lungs structural components were studied using method of lectin-peroxidase technique. The set of lectins included: concanavalin A (ConA, ?DMan specific), peanut lectin (PNA specific for ?DGal(?1-3)DGalNAc), soybean (SBA, specific for a DGalNAc), wheat germ (WGA, specific for DGlcNAc, NeuNAc), elderberry bark (SNA, specific for Neu5Ac(?2-6)Gal/DGalNAc), crust of golden rain (LABA, specific for ?LFuc). It has been shown, that experimental hypothyroidism is associated with perivascular edema and lectin receptors modification in the lungs structural components, induced the immune processes activation, that resulted in increased number of the SBA- positive alveolar macrophages and BALT-associated LABA positive dendritic cells. Lectin SBA (specific for a DGalNAc) may be considered as one of markers of alveolar macrophages, lectin LABA (specific for ?LFuc) as a marker of BALT-associated dendritic cells.